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KMID : 0377619710200050441
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Korean Jungang Medical Journal
1971 Volume.20 No. 5 p.441 ~ p.448
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STUDIES ON THE GROWTH OF VIBRIO PARAHAEMOLYTICUS AND VIBRIO CHOLERAE
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Abstract
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Acute diarrhea diseases including cholera are important public health hazard in Korea, and early diagnosis is necessary for the prevention and effective control of these diseases. The isolation and subsequent differentiation of Vibrio cholerae and Vibrio parahaemolyticus from clinical specimens are bacteriologically not difficult problem. However, it is sometimes neccessary to enrich the vibrios, especially when small number of vibrios are present in feces of very early or later stages of diseases and of carriers. In order to know the favorable conditions for the enrichment of vibrios, some experiments were carried out with the following results.
1. V. cholerae and V. parahaemolyticus grow well in the presence of 2.0 and 3.0% of sodium chloride in 1. 0% peptone water, but Escherichia coli grow well in the presence of 0.5-1.0% of sodium chloride.
2. V. cholerae show abundant growth at pH 6.5 through 8.5, and E. coli and V.parahaemolyticus at pH 6.5-7.0.
3. In general V. cholerae grow very luxuriously, V. parahaemolyticus moderately, and Ei coli slowly.
4. In mixed culture in 1.0% peptone water, V. cholerae overgrow E. coli within 6-24 hours of incubation, and this tendency is more marked at pH 8.5 than at 7.5. V. parahaen olyticus also overgrow E. coli, but the tendency is mild as compared with V. cholerae.
5. V.cholerae overgrow V. parahaeenolyticus in 1% peptone water, but the. rate of overgrowth is not so marked as in case of V. cholerae over E. coli.
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